# Study 000E Limitations

- This remains an `n=1` longitudinal study.
- The outside-context probe set is small (`5` later specialized outdoor runs).
- The burden claim is strongest at the `session level`.
- The next-day layer is less clean because several probe dates are clustered on consecutive days.
- The treadmill-versus-outdoor contrast remains a practical proxy for narrowed successful context versus outside-context expression, not a full environmental ladder.
- The study does not prove exact physiology mechanism.